Title:
E-cadherin expression in acquired cholesteatoma
Authors:
M. Erdoglija , N. Milanovic , M. Colic and M. Jovic 3
Institutions:
1Department of Otorhinolaryngology, Otology Unit, Military Medical Academy, Belgrade, Serbia; Institute of Medical2 Research, Division of Clinical Immunology, Military Medical Academy, Belgrade, Serbia; 3Institute of Pathology, Military Medical Academy, Belgrade, Serbia
Keywords:
E-cadherin, cholesteatoma matrix, antigen-presenting cells, Langerhans cells, immunohistochemistry
Pages:
241 - 246
Abstract:
E- cadherin expression in acquired cholesteatomaO . bjectives: The epithelial cell adhesion moleculeE-cadherin is important for maintenance normal tissue architecture and for cell-cell communication and immune cell migration. E-cadherin is also present in cholesteatomas. This study determined E-cadherin expression in acquired cholesteatomas and analyzed its expression according to cholesteatoma clinical and histological characteristics. Methods: We investigated E-cadherin expression in 30 samples from operated patients with acquired middle ear ­cholesteatomas­that­were­classified­according­to­their­clinical­and­histological­characteristics. E-cadherin expression in cholesteatoma was determined immunohistochemically. A semi quantitative method was used to determine the index of expression of E-cadherin and t-tests, Mann-Whitney U tests and Spearman correlation analysis were used for statistical analysis. Results: We­ found significant expression of E-cadherin on CD1, CD3 total, CD4 (p ­ ­ <0.05),­ high expression of E-cadherin ­ on CD8 total and CD19/CD38 lymphocytes (p ­ <0.01)­ and very high expression of ­ ­E-cadherin­ ­ on mast cells and antigen- presenting cells, including Langerhans cells (p ­ <0.005).­ We graduated results as no statistically significant (p>0.05), ­ ­tatistically­significant (0.05 >p>0.01),­ highly statistically significant (0.01 >p>0.005)­ and very highly statistically ­ ­ignificant­(p<0.005). Conclusion: E-cadherin expression was the same in the cholesteatoma matrix in all samples. There were no differences in expression according to the clinical and histological characteristics of the cholesteatomas.
Issue:
Vol. 9, 2013, 3rd trimester


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E-cadherin expression in acquired cholesteatoma