E-cadherin expression in acquired cholesteatoma
M. Erdoglija , N. Milanovic , M. Colic and M. Jovic 3
1Department of Otorhinolaryngology, Otology Unit, Military Medical Academy, Belgrade, Serbia; Institute of Medical2 Research, Division of Clinical Immunology, Military Medical Academy, Belgrade, Serbia; 3Institute of Pathology, Military Medical Academy, Belgrade, Serbia
E-cadherin, cholesteatoma matrix, antigen-presenting cells, Langerhans cells, immunohistochemistry
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E- cadherin expression in acquired cholesteatomaO . bjectives: The epithelial cell adhesion moleculeE-cadherin is important for maintenance normal tissue architecture and for cell-cell communication and immune cell migration. E-cadherin is also present in cholesteatomas. This study determined E-cadherin expression in acquired cholesteatomas and analyzed its expression according to cholesteatoma clinical and histological characteristics. Methods: We investigated E-cadherin expression in 30 samples from operated patients with acquired middle ear ­cholesteatomas­that­were­classified­according­to­their­clinical­and­histological­characteristics. E-cadherin expression in cholesteatoma was determined immunohistochemically. A semi quantitative method was used to determine the index of expression of E-cadherin and t-tests, Mann-Whitney U tests and Spearman correlation analysis were used for statistical analysis. Results: We­ found significant expression of E-cadherin on CD1, CD3 total, CD4 (p ­ ­ <0.05),­ high expression of E-cadherin ­ on CD8 total and CD19/CD38 lymphocytes (p ­ <0.01)­ and very high expression of ­ ­E-cadherin­ ­ on mast cells and antigen- presenting cells, including Langerhans cells (p ­ <0.005).­ We graduated results as no statistically significant (p>0.05), ­ ­tatistically­significant (0.05 >p>0.01),­ highly statistically significant (0.01 >p>0.005)­ and very highly statistically ­ ­ignificant­(p<0.005). Conclusion: E-cadherin expression was the same in the cholesteatoma matrix in all samples. There were no differences in expression according to the clinical and histological characteristics of the cholesteatomas.
Vol. 9, 2013, 3rd trimester

E-cadherin expression in acquired cholesteatoma