Title:
Gentamicin affects connexin 26 expression in the cochlear lateral wall
Authors:
P. Hu , R. Lai and D. Xie 1
Institutions:
Istitute of Otology, Department of Otolaryngology and Head and Neck surgery, Second Xiangya Hospital, Central South University, Changsha 410011, China
Keywords:
Gentamicin; ototoxicity; connexin 26 (Cx26); lateral wall
Pages:
77 - 84
Abstract:
Gentamicin affects connexin 26 expression in the cochlear lateral wall. Objectives/Hypothesis: Aminoglycosides may decrease the expression of some proteins participating in ion-exchange in the cochlear lateral wall. Connexin 26 expression in the lateral wall may play a role in acquired hearing loss by maintaining the endocochlear potential and potassium concentration in the endolymph. We examined the effects of gentamicin on the expression of connexin 26 to obtain a better understanding of aminoglycoside ototoxicity. Methods: We detected changes in connexin 26 protein and mRNAexpression in the cochlear lateral wall using immuno- histochemistry staining, western blotting, and real-time PCR in rats after administration of a single dose of gentamicin. Results: The expression of connexin 26 increased over time in type III fibrocytes after gentamicin administration. Elevated protein levels were detected 3 h after the single injection of gentamicin; while, mRNA levels increased after 24 h. Conclusion: Connexin 26 plays an important role in the acute effects of high-dose gentamicin and is probably involved in the pathogenesis of ototoxic deafness.
Issue:
Vol. 8, 2012, 2nd trimester


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Gentamicin affects connexin 26 expression in the cochlear lateral wall