Recruitment and activation of circulating neutrophils after sinus surgery
J.-B. Watelet , B. Chatelain , Ph. Eloy , J.-M. Dogne and F. Mullier
1Department of Otorhinolaryngology, Ghent University Hospital, Belgium; 2Laboratory of Hematology-Namur Thrombosis and Hemostasis Center, CHU Dinant-Godinne UCL Namur, Belgium; Department of Otorhinolaryngology, Head and Neck Surgery, CHU Dinant-Godinne UCL Namur, Belgium; Department of Pharmacy. Namur Thrombosis and Hemostasis Center, University of Namur, Belgium
Wound healing; neutrophils; cell migration; oxidative stress; neutrophil activation
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Recruitment and activation of circulating neutrophils after sinus surgery. Objective : After failure of pharmacological treatment, sinus surgery is the recommended alternative treatment for chronic sinusitis with or without nasal polyps. During post-operative healing, adequate local neutrophil activation plays an important role in the repair process. This pilot study aimed to systematically explore the participation of circulating neutrophils in early-phase wound repair of the nasal and paranasal mucosa after sinus surgery, with a special focus on neutrophil recruitment and activation patterns. Methodology: We conducted a single-center outcome study of patients undergoing sinus surgery. Whole blood samples were collected from eleven patients before surgery and at post-surgical time points of 1 hour and 1, 7, 14, and 30 days. Hematological analysis was conducted to count circulating neutrophils and evaluate their overall activation status. Using flow cytometry, neutrophil expression of membrane CD11b, CD11c, and CD15 was also measured, and oxidative burst analysis was performed. Results: After sinus surgery, neutrophilia increased by 1 hour after surgery, reached a maximum at Day 1, and showed a gradual return toward baseline by Day 30. The oxidative burst initially decreased during the first hours after surgery, increased at Day 14, and returned toward normal by Day 30. Lewis X factor and the expression of CD11b and CD11c exhibited a bimodal change over time, in an inverted phase compared to the oxidative reaction. Conclusions: Circulating neutrophils are involved in the first phase of wound healing after sinus surgery as indicated by increased abundance, early membrane changes, and the modulation of their oxidative capacities.
Vol. 11, 2015, 1st trimester

Recruitment and activation of circulating neutrophils after sinus surgery