Regulation of expression of galectin-7 in human nasal polyps by budesonide
C. Delbrouck*, C. Souchay*, H. Kaltner**, S. André**, H.-J. Gabius**, G. Vandenhoven*** and S. Hassid*
*E.N.T., Erasmus University Hospital, and **Institute of Physiological Chemistry, Faculty of Veterinary Medicine, Ludwig-Maximilians-University, Munich, Germany, ***Astra-Zeneca, Brussels, Belgium
Allergy; apoptosis; galectin-7; glucocorticoid; polyposis
137 - 144
Regulation of expression of galectin-7 in human nasal polyps by budesonide. Background: Nasal polyposis is a model for the study of inflammatory processes. We analyzed the expression of galectin-7, a growth regulator, in surface epithelium, glandular epithelium, and connective tissue in human nasal polyps, and examined the effect of the gluco- corticoid budesonide on its expression in human nasal polyps ex vivo. Methods: Using quantitative, computer-assisted microscopy and immunohistochemistry, we measured galectin-7 expres- sion in nine nasal polyps obtained by surgical resection. Five polyps came from allergic patients and four came from non-allergic patients. Results: Galectin-7 was expressed in all three polyp tissues analyzed. Treatment of polyps from allergic and non-allergic patients with 50 ng/ml budesonide increased the extent of galectin-7 expression in the connective tissue (p = 0.01). Conversely, budesonide at this concentration did not apparently affect galectin-7 expression in glandular epithelium; only a slight decrease in the percentage of the galectin-7-immunopositive cells was observed. In the surface epithelium of nasal polyps from non-allergic patients, the percentage of galectin-7- immunopositive cells was decreased (p = 0.03) by treatment with 250 ng/ml budesonide. In nasal polyps from allergic patients, this percentage was increased by treat- ment with 50 ng/ml budesonide (p = 0.0001). Conclusions: These data are consistent with a role for galectin-7 in the regulation of cell growth through a pro-apopto- tic effect. Galectin-7 expression coincides with the degree of epithelial stratification, and is subject to upregulation in the connective tissue in response to treatment with 50 ng/ml budesonide. Budesonide modulates galectin-7 expression dif- ferently in the surface epithelia of polyps from allergic and non-allergic patients.
Vol. 1, 2005, 3rd trimester

Regulation of expression of galectin-7 in human nasal polyps by budesonide