Effects of caffeic acid phenethyl ester on cisplatin ototoxicity

Purpose: To report histological and electrophysiological data in rats treated with cisplatin and caffeic acid phenethyl ester. Methods: We randomly divided 28 Wistar rats into four groups of seven, to be treated as follows: control (saline), cisplatin, CAPE and cisplatin-CAPE. Distortion product otoacoustic emission (DPOAE) measurements were performed on day one (before drug administration) and day five under anaesthesia. All animals were killed under general anaesthesia on day five after the DPOAE measurement. The cochleae of each rat were histopathologically and immunohistochemically evaluated. Results: The outer hair cells were mostly preserved in the control and CAPE groups. Moderate-to-severe and mild-tomoderate hair cell losses were detected in the cisplatin and cisplatin-CAPE groups, respectively. DPOAE assessments revealed significant deterioration in the cisplatin group (P < 0.05). The difference between the cisplatin and cisplatinCAPE groups was statistically significant (P < 0.05). Conclusion: CAPE prevents cisplatin ototoxicity